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Exemplary hybridization requirements having quick probes and you may primers is approximately 5 to help you a dozen values C An individual-feet mismatch inhibits DNA amplification and no detectable PCR product is molded , below the calculated Tm. Formulas for calculating Tm are known and include: Tm=4° C.?(number of G's and C's in the primer)+2° C.?(number of A's and T's in the primer) for oligos <14 bases and assumes a reaction is carried out in the presence of 50 mM monovalent cations. Read More
